Similarly, the presence of subgingival plaque containing gram-positive bacteria may be necessary for its secondary colonization in periodontal pockets. View Show abstract
Subgingival plaque samples presented significantly higher counts of Prevotella nigrescens, Prevotella intermedia, Bacteroides forsythus and Porphyromonas gingivalis. Subgingival samples exhibited a significantly higher proportion of “red” and “orange complex” species, while supragingival plaque exhibited higher proportions of “green” and “purple” complex species as well as
Section of Periodontology and Division of Stomatology, School of Dental and Oral Surgery, Columbia University, New York. The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface. BACKGROUND: The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. Subgingival plaque sample collection. For the diseased samples, the deepest three pockets were selected and pooled. Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze.
Three paper points were inserted for 15 s and immediately removed to a microcentrifuge tube in 500 μl of Tris-buffered saline. A total of 1,170 samples of supra and subgingival plaque were collected from the mesial aspect of every tooth (up to 28 supra and 28 subgingival samples) from each subject and evaluated for the was followed by collection of a pooled subgingival sample, collected from four sites with the deepest PD using the curette technique.26 Subgingival plaque samples were suspended in sterile saline (500 𝜇l). All samples were placed on dry ice immediately after collection followed by storage at −80 C until further analysis. Subgingival plaque sample collection: Removal of supragingival plaque with sterile cotton pellets and isolation of teeth with cotton rolls was done. Subgingival plaque was then collected with sterile paper points (Meta Biomed ® Co. Ltd, Cheongju, South Korea) inserted into the gingival sulcus of Ramfjord’s index teeth for 15 seconds(7-9).
Subgingival plaque was frequently characterized by a zone of gram negative and/or motile species located adjacent to the epithelial lining of the pocket while gram positive 2016-01-01 Background: The collection of subgingival plaque samples with paper points is time‐consuming and accident‐sensitive.
Sample Collection. Buccal and subgingival plaque samples were collected 1 week after the full-mouth periodontal examination. All participants were requested to refrain from food for 8 h and oral hygiene (brushing or flossing the teeth) for 12 h before sampling. Samples from all subjects were collected and stored at −80°C for subsequent
Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently. The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria.
MINST has been introduced as a concept that aims at obtaining extensive subgingival debridement with a retention of the preoperative gingival architecture, creating a minimal wound, and gentle handling of the soft and hard tissues to stimulate the formation of a stable blood clot by natural filling of the infrabony defect [25-28].
Buccal and subgingival plaque samples were collected 1 week after the full-mouth periodontal examination. All participants were requested to refrain from food for 8 h and oral hygiene (brushing or flossing the teeth) for 12 h before sampling. Samples from all subjects were collected and stored at −80°C for subsequent In the present study, the supragingival plaque was not removed before collection of the subgingival plaque in order to simulate the conditions existing in periodontal offices when the patient is first examined. Bretz et al. (1989) demonstrated that the supragingival plaque does not interfere with the results of BANA hydrolysis. subgingival plaque can be successfully cultured, but the deeper layer may be missed [10].
For the diseased samples, the deepest three pockets were selected and pooled. Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently. Clinical examination of periodontal parameters and collection of saliva and pooled subgingival plaque samples from mesial-buccal sites of 4 first molars were performed before initial therapy and 2, 4 and 6 months respectively after mechanical therapy, and saliva samples were also collected 2 weeks after therapy. Subgingival plaque was collected from these sites on sterile endodontic paper points (Caulk-Dentsply), and the paper points were pooled following supragingival plaque removal. The samples were placed in 1.5-ml microcentrifuge tubes and frozen at −80°C until further analysis.
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Herbert I. Oshrain D.D.S. Section of Periodontology and Division of Stomatology, School of Dental and Oral Surgery, Columbia University, New York.
This then allows for effective subgingival plaque removal by the patient, which if done daily will help to prevent calculus formation (calculus removal will be discussed in detail in Chap. 5). 2014-08-05 · Subgingival plaque samples were taken from the mesio-buccal aspect of each tooth in each subject at each monitor-ing visit.
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The purpose of subgingival scaling and root planing is to remove calculus and to smooth the cementum and underlying dentin. This then allows for effective subgingival plaque removal by the patient, which if done daily will help to prevent calculus formation (calculus removal will be discussed in detail in Chap. 5).
Section of Periodontology and Division of Stomatology, School of Dental and Oral Surgery, Columbia University, New York. The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface. At baseline, measurements of site-specific periodontal parameters and collection of subgingival plaque sample were done. After full-mouth SRP, subgingival delivery of 2% curcumin gel in experimental sites and 0.2% chlorhexidine gel in control sites was done. Subgingival plaque and placental biopsy were studied for Porphyromonas gingivalis, Fusobacterium nucleatum, Treponema denticola, Prevotella intermedia and Aggregatibacter actinomycetemcomitans using quantitative polymerase chain reaction technique. Periodontist and laboratory personnel were unaware of case or control status. Four samples (subgingival and supragingival plaques, saliva, and tongue coating) per each subject were collected from 14 patients with a broad range of severity of periodontitis before periodontal therapy.
3 Apr 2017 Four samples (subgingival and supragingival plaques, saliva, and tongue coating) per each subject were collected from 14 patients with a
Subgingival plaque sample collection. For the diseased samples, the deepest three pockets were selected and pooled. Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently.
The samples were immediately placed at 4 C and analyzed within 4 h. Subgingival plaque samples from 259 adult patients with severe periodontitis were analyzed. P. gingivalis was detected in 111 (43%) of the 259 subgingival plaque samples by culture and in 138 (53%) samples by PCR. The sensitivity, specificity, and positive and negative predictive values of the real-time PCR were 100, 94, 94, and 100%, respectively. Define subgingival plaque.